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Z10.02 - Analysis of Epithelial Cell Barrier Function in a Three Dimensional Culture Using an Organic Electrochemical Transistor 
Date/Time:
April 24, 2014   2:00pm - 2:15pm
 
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Cellular lines models are widely used to study cell behavior under different conditions. Epithelial cells are the most analyzed in vitro system, especially when they are grown as monolayers over a solid surface in two dimensional cultures. In these conditions, the cells keep many of the original characteristics; however recent reports show that the use of solid matrix to growth cells, or three dimensional (3D) cultures, mimics tissue characteristics, so the response to a specific stimulus is different than in monolayer, and more in vivo like. One of the protein complexes dedicated to sense these changes is the tight junction (TJ), which is localized in the most apical region of the epithelial cells. The main technique to analyze the barrier function of tight junction is the measurement of the transepithelial electrical resistance, which determines the ion flux through TJ; previously in our group an Organic Electrochemical Transistor (OECTs) was developed to accurately record the TJ barrier activity. In the present work we combine the 3D culture and the OECTs to analyze the tight junction in a 3D system such as kidney epithelial cyst. We determined the optimum conditions of the cyst culture in a Matrigel matrix, the biochemical characterization, as well as the barrier properties of the tight junctions under different stimuli.
 


 
 
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